84-1081-26　Sarcoglycan, delta 100ug　S0160

特徴

• Delta-sarcoglycan is one of six known sarcoglycans, which are type I (alpha and epsilon) or type II (beta, gamma, delta or zeta) transmembrane glycoproteins.
• Sarcoglycans associate in different combinations in a tissue-specific manner, to form heterotetrameric, pentameric and hexameric membrane glycoprotein complexes (SGC).
• SGC interacts laterally with the dystroglycan complex, and both complexes are components of the dystrophin-associated glycoprotein complex (DGC), which forms a link between the cytoskeleton and the extracellular matrix.
• Mutations in sarcoglycans are associated with recessive autosomally inherited muscular dystrophy.
• Applications:Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Direct ELISA: 0.5-1.0ug/ml; Detection limit is ~0.3ng/wellWestern Blot: 0.1-0.2ug/ml; Detection limit is ~5ng/lane (non-reducing) and 1ng/lane (reducing)Immunohistochemistry: 10ug/ml; Detects delta-Sarcoglycan in human fetal heart tissue sections.
• Sections were fixed with PBS, 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT.
• After blocking, cells were incubated with diluted S0160 overnight at 4°C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for 1 hr.
• Between each step, cells were washed with PBS, 1% BSA
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.