Vaccinia virus (VACV or VV) is a large, complex enveloped virus belonging to the poxvirus family of viruses.
It has a linear double-stranded DNA genome, which is approximately 190kb in length and encodes for approximately 250 genes.
The dimensions of the virion are roughly 360 × 270 × 250nm.
Vaccinia virus is well-known for its role as a vaccine that eradicated the smallpox disease from our world, making it the first human disease to be successfully eradicated by mankind.
This endeavor was carried out by the World Health Organization under the Smallpox Eradication Program.
Post eradication of smallpox, scientists have been studying vaccinia virus to use as a tool for delivering genes into biological tissues (gene therapy and genetic engineering).
Moreover, due to recent concerns about smallpox resurfacing as a possible agent for bioterrorism, scientists have renewed their interests in studying vaccinia virus.
Applications:Suitable for use in ELISA, Fluorescence Microscopy, Immunocytochemistry and PRNT (Plaque-Reduction Neutralization Test).
It is also suitable for Conjugation purposes.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
End-Point Titer:>1:1000 by indirect ELISA
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：rabbit
Grade：Highly Purified
Purity：Purified IgG fraction to a purity of ≥95%.
Form：Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins added. No preservative added. Also available with azide. See V0500-11.
Specificity：Recognizes Vaccinia, purified virions. Recognizes the B5R and the A33R proteins. Species Crossreactivity: Lister, New York City, MVA strains of Vaccinia and Monkeypox. Does not crossreact with Parainfluenza (1-3), RSV, Adeno, Influenza A & B, HSV (1) or uninfected cells.
Isotype：IgG
Calc Applications Abbrev：E IC IF
Immunogen：New York City Board of Health strain (NYCBOH).
