The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone.
P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase.
There are over 200 known genes which encode P450s.
Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20- hydroxyeicosatetraenoic acids (19- and 20-HETE).
As well as fatty acid metabolism, P450s also metabolize many drugs and toxins.
Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
Phase 1 drug metabolism is catalyzed by two groups of monooxygenases, the cytochrome P450s (CYPs) and the flavin monooxygenases (FMOs).
Both CYPs and FMOs are localized in the endoplasmic reticulum.
Collectively, these enzymes catalyze the oxidation of a very broad range of substrates.
Both classes are dependent on NADPH as a source of electrons, but the two classes exhibit significant differences in mechanism and substrate specificity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions: Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS. Labeled with Biotin. No preservative and no stabilizing protein added.
Specificity：Recognizes human cytchrome P450 (3A4 isoforms). Weakly cross-reacts with other P450 isoforms. Species crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Purified full length human cytochrome P450 (isoform 3A4) fusion protein.
