Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:100Immunohistochemistry (Bn-SA/HRP Technique): 1:100-1:300 in PBS.
Immunohistochemistry (Bn/Av-HRP Technique): 1:500-1:1000 in PBS.
Optimal dilutions to be determined by the researcher.
Immunohistochemistry:Perfusion Fixation: Fixative-4% paraformaldehyde in 0.1M PBS, pH 7.4; 500ml over ~20 minutes.
Post Fixation: 4% paraformaldehyde in 0.1M PBS, pH 7.4; 500ml over ~20min at RT or 1.5hr at 4°C.
Note: Paraformaldehyde is a necessary component in fixation of serotonin.
If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde
Sections: 50um vibratomeAdsorption control: Synthetic peptide corresponding to aa8-23 of the rat 5-HT7 receptorIncubation on Tissue: 16-24 hours at 4ºC.
Positive Control:Rat cortex and hippocampus tissues
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid.
Specificity：Recognizes rat Serotonin Receptor 7. Species Crossreactivity: mouse and human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa8-23 of the rat 5-HT7 receptor
