84-1080-87　［取扱停止］Calcitonin Gene-Related Peptide (CGRP) 50ul　C0115-01G

特徴

• Calcitonin gene-related peptide (CGRP) is present in C-cells of the thyroid and in central and peripheral nerves.
• CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: 1:100-1:500.
• Used on perfusion fixed tissue.
• Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant.
• Desired tissues were dissected and stored overnight in 10% sucrose in PBS.
• Slide-mounted tissue sections were processed for indirect immunofluorescence.
• Slides were incubated with blocking buffer for 1 hour at RT.
• C0115-01G was diluted with blocking buffer.
• Blocking buffer was removed from slides and they were then incubated for 18-24 hours at 4ºC with diluted C0115-01G.
• Slides were rinsed 3x and then incubated with secondary antibody for 1 hour at RT.
• Slides were again rinsed 3x and coverslipped.
• Staining was examined using fluorescence microscopy
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1906: IgY, Chicken Pab Rb x Ch I1906-10: IgY, Chicken-HRP Rb x Ch I1906-12: IgY, Chicken-FITC Rb x Ch I1906-14: IgY, Chicken-AP Rb x Ch
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.