MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated proteinkinase-2.
This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro.
While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of theresidues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay.
MK2 is serine-threonine protein kinase that contains a proline rich sequence and twoputative SH3-binding sites.
MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif.
The bestcharacterized MK2 substrate is hsp27.
Although unknown, thephysiological function of MK2 may be to increase stress resistance.
MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNF-a at a posttranscriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.
See Control Peptide M2355-01A1
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes MAPKAP. Detects a single 44kD protein as indicated in crude extracts prepared from either unstimulated or TNFα stimulated human HeLa cell lysates. This sequence is conserved 100% in human, mouse, rabbit, rat, hamster, chicken, Xenopus and zebrafish.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa 310-325 of MAPKAP Kinase 2 (MTSALATMRVDYEQIK) conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
