Endothelial Cell Differentiation Gene-6, or EDG-6, encodes a 45kD protein belonging to a family of G-Protein Coupled Receptors (GPCR’s), which bind lysophospholipids.
To date, 8 members of the EDG receptor family have been identified.
These receptors are suggested to have a role in modulation of growth-related effects including cell proliferation and differentiation as well as protective, anti-apoptotic effects.
EDG receptor expression is developmentally regulated and the expression of the 8 members of this family of proteins appears to be tissue specific.
EDG receptors couple to G-proteins to signal through Ras and MAPK pathways and other protein tyrosine kinase pathways.
EDG-6 is also known as S1P4.
The ligand for EDG-6 is sphingosine-1-phosphate.
EDG-6 is expressed by lymphoid and hematopoietic tissues and also by lung tissue.
The biological function of EDG-6 is currently under investigation.
Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8.
S1P3 couples to Gi/o-ERK, Gq-PLC, and G12/13-Rho axes to mediate S1P-induced cell proliferation, survival, migration, and related signaling events.
S1P3 is widely expressed in various tissues, suggesting diverse physiological functions of this receptor.
The human and murine S1P3 have 378 amino acids with an estimated molecular weight of 42kD.
Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at different positions in SDS-PAGE.
Cayman’s S1P3 polyclonal antibody detects the receptor at 47kD using samples from human cerebral cortex.
Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degree of glycosylation.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 9.6ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.7, 0.01% sodium azide.
Specificity：Recognizes EDG 6 in (Endothelial Cell Differentiation Gene 6, S1P4), human cerebral cortex. Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.
