Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8.
S1P3 couples to Gi/o-ERK, Gq-PLC, and G12/13-Rho axes to mediate S1P-induced cell proliferation, survival, migration, and related signaling events.
S1P3 is widely expressed in various tissues, suggesting diverse physiological functions of this receptor.
The human and murine S1P3 have 378 amino acids with an estimated molecular weight of 42kD.
Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at different positions in SDS-PAGE.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:200Immunocytochemistry: 1;100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.4, 5mg/ml BSA.
Specificity：Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa12-25, VRGNETLREHYQYV of human EDG 3 protein
