Description:Tumor necrosis factor (TNF) receptor associated factors (TRAFs) were initially discovered as adaptor proteins that link the TNF receptor superfamily to signaling pathways and are thus important regulators of cell death and cellular response to stress (1).
TRAF proteins share a homology region that allows them to bind to cell receptors and other TRAF proteins, causing the activation of different signal cascades depending on the TRAFs involved.
For example, TRAF2 and TRAF3 directly bind to the CD40, a TNF receptor superfamily member involved in inducing B cell immunity (2), and are critical for NF-κB activation in mouse B lymphocytes (3).
TRAF2 along with TRAF6 has also been shown to be required for CD40 signaling in nonhemopoietic cells (4).
TRAF2 also interacts with the TRFR superfamily member lymphotoxin-β receptor (LTβR) in association with TRAF3 and the apoptosis inhibitors cIAP1 and Smac (5).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug’mlOptimal dilutions to be determined by the researcher.
Positive Control:Mouse liver tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human TRAF2. Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide TRAF2 17aa near the carboxy terminus of human TRAF2 (Genbank accession No. CAI15106).
