Description:Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila.
These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2).
The TLRs act through adaptor molecules to activate various kinases and transcription factors (3) so the organism can respond to potential infection.
These adaptor molecules include TOLLIP, MyD88, and TRIF.
TOLLIP associates directly with TLR2 and TLR 4, acting as an inhibitor to TLR activation (4).
This negative regulation of TLR signaling may serve to limit the production of proinflammatory mediators during infection and inflammation.
Applications:Suitable for use in Western Blot and Immunohistochemistry,
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Rat Brain cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human TOLLIP. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide TOLLIP 16 aa near the carboxy terminus of human TOLLIP (UniProt # P60484).
