84-1080-49　［取扱停止］PMAIP1 (Phorbol-12-Myristate-13-Acetate Induced Protein 1, APR, NOXA) 100ug　P4285-52

特徴

• Apoptosis is related to many diseases and development.
• The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA.
• A new p53 target gene, Noxa, was recently identified (1), which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins.
• Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins.
• X-ray irradiation increased p53-dependent Noxa mRNA and protein levels.
• Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9 (1).
• Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53 (1-3).
• Noxa and PUMA may represent direct mediators of p53-induced apoptosis.
• Increased levels of p53 and its target gene Noxa was found in the impaired tumor development (4).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry: 1ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Human stomach cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.