Description:Apaf-1 and NOD1 are members of a new family (1), which are involved in the regulation of apoptosis and immune response.
Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD).
A third member in this family was recently identified and designated NOD2 (2).
NOD2 interacts with RICK via a homophilic CARD-CARD interaction.
NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria.
The variants of NOD2, either a frameshift or a missense, were associated with Crohn’s disease (3,4) that is a main type of chronic inflammatory bowel disease.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2-4ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human NOD2.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide, 16aa at the amino terminus of human NOD2 (GenBank accession no. Q9HC29).
