Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells.
It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3).
A protein recently found to be involved in autophagy, LAMP- 2, is a highly glycosylated protein associated with the lysosome (4,5).
LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions.
Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression (6).
However, the loss of both proteins resulted in embryonic lethality (7), suggesting that each protein possesses some unique and necessary functions.
Applications:Suitable for use in Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:EL4 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human LAMP-1. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：15aa synthetic peptide near the center of human LAMP-1 (Genbank accession No. NP_005552).
