Description:Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins.
LSD1 is a recently discovered amine oxidase that catalyzes the lysine- specific demethylation of histone proteins via an FAD- dependent oxidative reaction (1).
Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2).
LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3- K4-specific methylase complex that is involved in transcriptional regulation (3,4).
Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3).
LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug’mlOptimal dilutions to be determined by the researcher.
Positive Control:P815 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human LSD1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide LSD1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human LSD1 (Genbank accession No. NP_001009999).
