Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94 (1,2).
These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 (3,4) but ER stress causes their dissociation.
This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus.
IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation (5).
Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER (6).
Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human IRE1p.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：15 amino acid peptide from near the amino terminus of human IRE1p (UniProt # O75460).
