Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells.
It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2).
This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3).
Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin- E1-like enzyme; this function is conserved in the mammalian homolog (4).
In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5).
It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6).
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunocytochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:L1210 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human APG7. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide, 17 amino acid from near the amino terminus of human APG7 (Genbank accession No. NP_006386).
