WW domain-containing oxidoreductase WWOX, also known as WOX1 or FOR, is a proapoptotic protein and a tumor suppressor gene.
Because mutation or deletion in the coding region of WWOX is rarely found, it is speculated that the appearance of aberrant transcripts affects progression of many cancers.
In addition, protein levels of WWOX, its Tyr33-phosphorylated form, and WOX2 are significantly down-regulated in the neurons of Alzheimer's hippocampi.
Applications:Suitable for use in Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Note: Investigators occasionally observe in overdeveloped blots a faint upper ladder-like banding pattern in many cell types using WWOX1 antibodies against Tyr33 phosphorylation.
It is surmised that faint upper bands may represent WWOX ubiquitination.
However these bands do not appear if properly developed.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Specific for the peptide representing residues 89-107 RLAFTVDDNPTKPTTRQRY of WWOX. Reacts with human and mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide representing residues 89-107 RLAFTVDDNPTKPTTRQRY of WWOX.
