The novel BRCA1 protein’s epitope, SSKKQKRSHK, was mapped to the cytoplasm of MCF-7 cells.
munohistochemistry analysis showed that the antigen epitope exhibited positive expression in 65% of breast cancer specimens.
Its expression is associated with characteristics that are associated with prognosis of breast cancer, and its sera antibody level may be helpful for breast cancer diagnosis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human residues 1046-1055 (SSKKQKRSHK) of the 136kD BRCA1 protein. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide aa1046-1055 (SSKKQKRSHK]) of the human 136kD BRCA1 protein.
