Applications:Suitable for use in ELISA, Western Blot, Neutralization and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1ug/ml Neutralization: The neutralization dose is 0.2-1ug/ml in the prescence of 0.03ug/ml of rhCXCL5Immunohistochemistry (FFPE): 5-15ug/ml in fixed paraffin-embedded section of human breast and human breast cancer tissueOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/1ug
Form：Supplied as a lyophilized powder in PBS, pH 7.4, 5% Trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human Lipopolysaccharide-induced CXC Chemokine. Neutralizes human CXCL5 bioactivity. In Direct ELISAs, ~20% cross-reactivity with recombinant human GCP-2 and <5% with recombinant mouse KC, recombinant rat CINC-1, recombinant human GROa, recombinant human GROy and recombinant mouse GCP-2.
Isotype：IgG
Calc Applications Abbrev：E IHC N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa37-114 of human Lipopolysaccharide-induced CXC Chemokine expressed in E. coli (P42830).
