Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans.
Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely beta-sheet structure.
Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.
In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.
The genes encoding human alpha and beta-defensins are clustered in a contiguous segment of chromosome 8p23.
Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides.
beta-Defensins are slightly larger and differ in the position and arrangement of 3 disulfides.
In humans, six alpha-defensins (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two beta-defensins, HBD-1 and HBD-2, have been identified
Alpha-defensins are encoded by genes designated DEFA1-6, whereas human beta-defensins are encoded by the DEFB1 and DEFb2 genes.
HD1-4 are expressed in neutrophils, whereas HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract.
HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein.
The HNPs are 29 to 30 amino acids long and are identical in sequence except at the N-terminal amino acid.
DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively.
DEFA2 is identical to both except that it has 29 instead of 30 amino acids
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested
Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml.
Optimal dilutions to be determined by the researcher
Control Peptide: N5378-07
Storage and Stability:Store at 4°C.
Do Not Freeze.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Note: Applications are based on unconjugated antibody.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative and no stabililizing protein added. Labeled with HRP.
Specificity：Recognizes human alpha defensin-5. No significant homology exists with other alpha-defensins.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A synthetic 17aa peptide sequence near the C-terminus of human mature alpha defensin-5 (KLH).
