The Janus family tyrosine kinases, consisting of Jak1, Jak2, Jak3 and Tyk2, associate with a variety of cytokine receptors and are activated by ligand binding to these receptors (1).
Upon ligand binding to cytokine receptors, Jaks phosphorylate themselves and their associated receptors, thereby providing multiple binding sites for signaling proteins containing SH2 or other phospho-tyrosine-binding domains, including Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (5).
The tyrosine residues 1022/1023 of Jak1 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Also available with BSA & Azide. See J0900-20A.
Specificity：Recognizes endogenous levels of human Jak1 only when phosphorylated at tyrosines 1022/1023. Species Crossraeactivity: mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to residues around Tyr1022/1023 of human Jak1. Species sequence homology: rat and bovine.
