Applications:Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:250-1:1,250Optimal dilutions to be determined by the researcher.
Absorption Wavelength: 495nm.
Emission Wavelength: 528nm.
Fluorochrome/Protein Ratio: 2.5 moles FITC per mole of IgG.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 40% glycerol. No preservative added. No stabilizing proteins added. Labled with FITC. Also available with BSA & preservative. See I1904-45B.
Specificity：Recognzies human IgG, F(ab’)2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, human IgG, human IgG F(ab’)2 and human serum. Does not crossreact with Human IgG F(c).
Isotype：IgG
Calc Applications Abbrev：FLISA IF WB
Calc Crossreactivity：Hu
Immunogen：Human IgG F(ab’)2 fragment (cat# I1903-90A).
