Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.
TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions.
Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Endotoxin:≤.00005EU/ug protein.
Applications: ELISAWestern Blot: 10ng recombinant rat TNF-a by 40-60ug/ml.
Functional Application:Neutralizes cytotoxic activity of recombinant rat TNF-a (Neutralizing activity: >2x10e5 U/mg.
One unit is defined as the amount of antibody sufficient for neutralizing the cytotoxic activity of 1 unit recombinant rat TNF-a).
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Highly Purified
Purity：Highly Purified
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes human, mouse and rat TNFa.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to rat TNFa.
