84-1079-44　Prostaglandin D Synthase, Lipocalin form (PGD Synthase, L-PGDS) 500ul　P9053-24L

特徴

• Prostaglandin D synthase (PGDS) catalyzes the isomerization of PGH2 to produce PGD2.
• PGD2 induces sleep, regulates nociception, inhibits platelet aggregation, and acts as an allergic mediator.
• Two distinct types of PGDS have been identified, namely the lipocalin type enzyme (beta-trace) and the hematopoietic enzyme (1,2).
• Lipocalin-type PGDS (L-PGDS) is localized in the central nervous system, genital organs of various mammals, and the human heart (1,3-5).
• Patients with chronic renal failure and hypertension exhibit elevated amounts of L-PGDS in serum and urine (6).
• The L-PGDS has been identified as beta-trace, which is a major protein in the human cerebrospinal fluid (2,7).
• Human L-PGDS is a 190 amino acid protein and can be detected at 24-26kD by immunoblotting.
• Applications: Suitable for use in Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions: Immunohistochemistry: 1:2000Western Blot: 1:1000 Incubate for 1 hour at RT.
• Overnight incubations at 4ºC with greater dilutions have also produced optimal results
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.