Visfatin is pre-B cell colony-enhancing factor 1(PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice.
This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (Direct and Indirect): 1:2000-1:5000Western Blot: 1:2000-1:5000 using ECL.
Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk.
Incubation time: 1 hour at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified immunoglobulin
Form：Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity：Recognizes human Visfatin. Species Crossreactivity: Weakly crossreacts with mouse Visfatin.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa40-56, R40EKKTENSKLRKVKYEE56 of human Visfatin.
