84-1079-36　PARP 1 (Poly ADP-Ribose Polymerase 1) 100ul　P3113-20G

特徴

• Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells.
• Poly(ADP-ribose) polymerases (PARPs) now constitute a large family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113kD), the founding member, and PARP-2 (62kD) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks.
• A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death.
• Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.
• Applications:Suitable for use in ELISA, ImmunocytochemistryImmunohistochemistry, Immunoprecipitation and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Immunocytochemistry: 1:4000Immunohistochemistry: Frozen and paraffin sectionsImmunoprecipitation: 1:400Western Blot: 1:4000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.