5-Oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OxoETE) is a potent stimulator of chemotaxis for eosinophils and neutrophils that mediates its action via a putative G-protein coupled receptor (GPCR).
The 5-OxoETE receptor couples to Gi/o to inhibit cyclic AMP production and to mobilize intracellular calcium.
Northern blot analysis reveals that the receptor is highly expressed on eosinophils and neutrophils, suggesting a key role in the inflammatory response.
In addition, the protein can also be detected in tissues such as kidney and liver.
Applications:Suitable for use in Western Blot, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200Immunocytochemistry: 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.4, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human 5-OxoETE receptor. Species Crossreactivity: African green monkey, mouse, porcine and rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mk Mo Po Rt
Immunogen：Synthetic peptide corresponding to the C-terminal region of human 5-OxoETE receptor.
