84-1079-32　Optineurin, CT (FIP-2, NEMO-Related Protein, NRP) 500ul　O7037-02

特徴

• Optineurin (OPTN) has recently been linked to glaucoma, a major cause of blindness worldwide.
• Mutations in OPTN such as Glu50->Lys (E50K) have been reported in patients, particularly those with normal pressure glaucoma.
• Apoptosis is controlled by a variety of signalling mechanisms and is relevant to several diseases and normal development.
• Optineurin (originally named FIP-2) is a 74kD protein implicated in signal transduction of the tumor necrosis factor (TNF) pathway as presented in a study of adenovirus proteins that prevent cytolysis after TNF alpha stimulation (ref1).
• Optineurin can be phosphorylated, however it is currently unknown what kinases achieve this or what other mammalian proteins interact with it downstream of TNF alpha or Fas signalling
• Working at or near cell membranes and in concert with Rab8 and Huntingtin, optineurin has been implicated in membrane traffic regulation and cellular morphogenesis (ref 3).
• The exact role of optineurin protein in apoptosis is currently unknown and interestingly optineurin gene mutations have been correlated with certain types of glaucoma (ref 4-6).
• Increased apoptosis of trabecular meshwork cells is an identified factor for pathogenesis of glaucoma (ref 7).
• Positive controls include murine or human heart, brain, or fibroblast lysates.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:200.
• Incubate 1 hour at RT.
• Overnight incubations at 4ºC at lower concentrations can produce optimal results
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.