NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline.
Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and antipathogenic activities..
NOS is classified under three types: Type I NOS is 150-160kD and is found in neurons.
It is constitutively expressed and is Ca2+/calmodulin dependent.
It is called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral / constitutive / Ca2+regulated NOS (cNOS).
bOS is present in skeletal muscles and its absence in Duchenne’s muscular dystrophy accounts for its symptoms of the disease.
Type II NOS is 130kD protein known as macrophage NOS (mNOS) or inducible NOS (iNOS).
It is Ca2+/calmodulin independent and is expressed in activated macrophages and stimulated glial cells.
Type III NOS is a 135kD protein called endothelial NOS (eNOS).
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Detects a band of ~140kD
Immunocytochemistry: 1:250Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes eNOS (NOS III). Does not crossreact with inducible NOS (iNOS; NOS II) or neuronal NOS (nNOS; NOS I). Species Crossreactivity: human, mouse, rat and canine
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa598-612, PYNSSPRPEQHKSYKC, of bovine endothelial nitric oxide synthase.
