Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase.
NO reacts rapidly with superoxide (6.7 x 10e9M−1sec−1) to form peroxynitrite.
At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues.
Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.4, 0.5mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes nitrotyrosine. Less than 10% reactivity with chlorotyrosine. Does not cross-react with synthetic peptides containing unmodified tyrosine.
