Endocannabinoids, such as arachidonyl ethanolamide (AEA) and 2-Arachidonyl glycerol (2-AG), function as short-range modulators of cell and synaptic activity.
Monoacylglycerol lipase (MAGL) hydrolyzes 2-AG to terminate its biological actions and works consecutively with hormone-sensitive lipase (HSL) to mobilize fatty acids from the triglyceride stores of adipocytes.
MAGL has a molecular weight of ~33kD and exhibits a high degree of homology among human, mouse, and rat at the amino acid level.
MAGL is expressed in a variety of tissues such as kidney, spleen, heart, liver, testis, stomach, brain, lung, and adrenal gland, with most abundant expression in skeletal muscle and adipose tissue.
This suggests a role of MAGL in monoglyceride hydrolysis in diverse tissues.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:300; Band at 35kDImmunohistochemistry (formalin-fixed parrafin-embedded tissue): 1:150Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human monoglyceride lipase. Species Crossreactivity: Mouse, rat and bovine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human monoglyceride lipase.
