Munc18 is a cytosolic brain protein of ~67kD which plays a key role in vesicle membrane fusion events with the plasma membrane.
Functional interactions have been demonstrated to exist between Munc18, synaptosomal associated protein of 25kD (SNAP-25), synaptobrevin, and syntaxin.
Munc18 is the mammalian homologue of the C.
elegans gene unc-18.
Mutations in Munc18 can result in accumulations of acetylcholine and paralytic phenotype suggestive of a neurotransmitter release defect.
Munc18 thus appears to be required by the synaptic vesicle fusion complex.
Two Munc18 isoforms have recently been identified.
Munc18-1 has been shown to be primarily expressed in brain, while Munc18-2 is expressed in most tissues.
Applications:Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlImmunohistochemistry: Frozen sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes Munc18 at ~67kD by Western Blot. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa58-70, VEDINKRREPIPS of human Munc18.
