MDA (Malondialdehyde) is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism.
MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA.
MDA-modified proteins may show altered physico-chemical behavior and antigenicity.
Antibodies to MDA will help to visualize the MDA-adducts.
Applications:Suitable for use in Western Blot and ELISA.
Immunohistochemistry, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000ELISA: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
