Lysophosphatidic acid (LPA) is an extracellular signaling lipid that evokes multiple biological functions including induction of platelet aggregation, smooth muscle contraction, and stimulation of cell proliferation and chemotaxis.
Lysophospholipase D (LysoPLD) was first discovered in 1999 as the enzyme responsible for generating LPA from lysophosphatidylcholine (LPC).
It was later revealed to be identical to an autocrine motility factor, autotaxin (ATX), which plays a role in tumor progression and metastasis.
LysoPLD/ATX mRNA is widely expressed with highest levels found in brain, ovary, lung, intestine, and testis.
Rat LysoPLD is composed of 885 amino acids with an estimated molecular weight of 101kD.
The protein is reported to be heavily glycosylated and thus its apparent size on SDS-PAGE may be run as high as 125kD.
Useful positive controls include cerebrospinal fluid, mouse ascites or seminal plasma.
Applications:Suitable for use in Western Blot, ELISA and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:200Immunofluorescence (IC): 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.4, 1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat LysoPLD. Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa573-588, KNKLEELNKRLHTKGS from rat Lysophospholipase D (LysoPLD).Species Sequence Homology: mouse; 100% (16/16aa), human and chimpanzee; 93.75% (15/16aa).
