Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents.
Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance.
One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure.
Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.
Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1:1000Immunohistochemistry (frozen sections): 1:50-1:200Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes mouse leptin. Species Crossreactivity: human, rat, porcine and sheep.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Po Rt Sh
Immunogen：Synthetic peptide corresponding to aa25-44, QKVQDDTKTLIKTIVTRIND of mouse leptin.
