IP3R1 is a second messenger for many growth factors, hormones, and neuro-transmitters.
Upon binding to the IP3 receptor (IP3R), IP3 triggers the release of intracellular, luminal calcium to the cytosol.
Functional IP3R is a homo- or heterotetramer of ~240kD glycoprotein subunits, that is structurally reminiscent of members of the voltage-gated ion channel superfamily.
IP3R protein is structurally and functionally related to one other important intracellular calcium-release channel, the ryanodine receptor.
The similarity between the two receptors continues at the physiological level owing to a physical association each receptor can have with the immunophilin protein, FKBP12.
Cytosolic calcium levels appear to be regulated by a feedback loop that starts with calcium activation of protein kinase C (PKC) and calcineurin, a protein phosphatase.
Phosphorylation of the IP3R by PKC causes an increase in IP3-mediated calcium release.
Concomitantly, the phosphatase activity of calcineurin is stimulated upon its association with the FKBP12-IP3R complex.
Calcium release is reduced when the PKC target site on the IP3R is dephosphorylated by calcineurin resulting in calcium oscillations.
Mammalian IP3R subunits are the product of three distinct genes that are widely expressed and differentially regulated.
Type-I IP3R has been detected in heart, liver, kidney, ovary, and Purkinje neurons of the cerebellum.
IP3R-II protein is found predominantly in the brain.
Isoform III expression has been shown to occur in pancreatic islets, kidney and the gastrointestinal tract.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry (Frozen): 1:250Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes human IP3R-I (weak) at ~240kD. Species Crossreactivity: mouse, rat and canine
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1829-1848 NKKKDDEVDRDAPSRKKAKE of the cytoplasmic C-terminal domain of human IP3RI. The sequence is identical in mouse and rat.
