Hydroxysteroid Dehydrogenase, 11-beta, Type-1 catalyzes the conversion of inactive cortisone to active cortisol in adipose tissue.
Its expression more closely parallels that of the glucocorticoid receptor than that of the mineralocorticoid receptor, to regulate the exposure of cortisol to glucocorticoid receptors.
mRNA and protein of this enzyme have been detected in a wide range of tissues including liver, kidney, testis, lung, heart and colon.
The enzyme’s expression is developmentally regulated, suggesting its importance in regulation of glucocoricoid action during embryonic development.
Over-expression of 11beta-HSD1 results in visceral obesity and metabolic syndrome including insulin-resistant diabetes, hyperlipidemia and hyperphagia.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200Immunohistochemistry (Formalin-fixed paraffin-emedded sections): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human 11beta-HSD1 at 32kD. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the an internal region of human 11β-HSD1.
