84-1079-09　Hepsin (TMPRSS1) 500ul　H2030-21

特徴

• Hepsin is a type II membrane-associated protein that has an extracellular proteolytic domain and exhibits low sequence homology to other known proteases (1,2).
• Hepsin overexpression is observed in the prostate, breast, kidney, and ovarian cancers and due to low homology to other known proteases may provide a unique target for pharmacological therapy (3-7).
• Hepsin is necessary for cell growth in vitro and may play a role in metastatic expansion for Factor VII activation (6,9).
• The observed molecular mass of hepsin by immunoblotting is 45.3kD.
• Cloning and characterization of murine and rat hepsin indicate 88% overall homology with human hepsin (10-12).
• Northern blotting reveals high levels of hepsin transcripts in liver (2,7).
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:200Immunohistochemistry: 1-2ug/ml; Formalin-fixed paraffin-embedded sections.
• Antigen retrieval results in higher background staining and is not recommended
• Optimal dilutions to be determined by the researcher.
• Positive Control:K562 cell lysate
• Storage and Stability:For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.