84-1079-01　Estrogen Receptor beta (ERb) 50ug　E3565-16M

特徴

• Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors.
• Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues.
• They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen.
• ERα and ERβ have been shown to be differentially activated by various ligands.
• Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes.
• Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.
• Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot.
• Other applications have not been tested.
• Recommended Dilutions: Immunofluorescence: 1:50-1:200Immunohistochemistry (Paraffin): 1-2ug/mlWestern Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.