Applications:Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry (frozen): 1-5ug/ml Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Specificity:Recognizes endogenous human ER alpha. Detects a band of ~64kD by Western blot. Does not cross-react with ER beta. Species sequence homology: This sequence is completely conserved in rat and mouse ER alpha and is highly conserved in sheep, porcine, canine, bovine and chicken ER alpha. Species Crossreactivity: Rat
Isotype:IgG
Calc Applications Abbrev:IC IHC WB
Calc Crossreactivity:Hu Rt
Immunogen:Synthetic peptide corresponding to aa1-21, M1TMTLHTKASGMALLHQIQGN21, of N-terminal human ER alpha (estrogen receptor alpha). Species Sequence Homolgy: Mouse