84-1078-96　［受注停止］Endothelial Lipase (EL) 500ul　E3010-36

特徴

• Endothelial lipase (EL) is a member of the triglyceride lipase gene family.
• It functions primarily as a phospholipase and has low triglyceride lipase activity.
• It has been shown to be a major genetic determinant for the concentration, structure, and metabolism of high-density lipoprotein, which protects against atherosclerosis (1,2).
• It was originally cloned from endothelial cells and was found to be expressed in a distinct and complementary tissue-restricted fashion, with high-level expression in the liver, placenta, lung, ovary, and macrophage(3).
• The wide spread distribution of this protein suggests that it plays a general role in lipid metabolism.
• Immunohistochemical studies demonstrate that EL is expressed in infiltrating cells such as macrophages within atheromatous plaques, in addition to endothelial and smooth muscle cells in non-atherosclerotic coronary arteries.
• Furthermore, EL expression is detected in the neovasculature within atheromatous plaques in atherosclerotic coronary arteries, indicating that EL may have unique functional roles in atherosclerosis.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 3ug/mlImmunohistochemistry (FFPE): 2ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Western Blot: HepG2 cell lysateImmunohistochemistry: Paraffin-embedded rat kidney sections.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.