84-1078-92　Cytochrome P450 3A4 (CYP3A4, CYP3A3, HLP, NF-25, P450C3, P450PCN1, Glucocorticoid inducible P450, Nifedipine Oxidase) 100ul　C9095-15N2

特徴

• The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone.
• P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase.
• There are over 200 known genes which encode P450s.
• Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20- hydroxyeicosatetraenoic acids (19- and 20-HETE).
• As well as fatty acid metabolism, P450s also metabolize many drugs and toxins.
• Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
• Phase 1 drug metabolism is catalyzed by two groups of monooxygenases, the cytochrome P450s (CYPs) and the flavin monooxygenases (FMOs).
• Both CYPs and FMOs are localized in the endoplasmic reticulum.
• Collectively, these enzymes catalyz e the oxidation of a very broad range of substrates.
• Both classes are dependent on NADPH as a source of electrons, but the two classes exhibit significant differences in mechanism and substrate specificity.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:2000 detects a band of ~46kDOptimal dilution determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.