COX catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2.
Recent discoveries of the induction of COX by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothosis that the inducible form of COX, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions.1,2 COX-2 is a 72kD protein which has been cloned from a variety of species including human, mouse, rat, and sheep.
Applications:Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution: Immunohistochemistry: 1:300Immunocytochemistry: 1:300Western Blot: 1:200Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1Ea
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse COX-2. Species Crossreactivity: human, rat, sheep, monkey and guinea pig COX-2. Does not cross-react with COX-1.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Gp Hu Mk Mo Rt Sh
Immunogen：Synthetic peptide corresponding to C-terminal aa570-598 (D570PQPTKTATINASASHSRLDDINPTVLIK598) of mouse/rat COX-2, conjugated to BSA. UniProt Accession: Q05769
