COX-2 has been cloned from a variety of species including human, rat, and sheep and has a molecular weight of 72kDa.1,2,3,4, COX-2 from these species are approximately 90% identical at the amino acid level, whereas the homology between COX-1 and COX-2 is only about 60%.
Expression of COX-2 is regulated by growth factors, tumor promoters, bacterial endotoxin and cytokines, including IL-1 beta.5,6 COX-2 is present in human breast tumors but is not expressed in normal breast tissue.7
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/ml (1:200)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1Ea
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified.
Form：Supplied as a liquid in TBS, pH 8.0, containing 50% glycerol and 0.02% sodium azide.
Specificity：Recognizes human COX-2. Species Crossreactivity: sheep. Does not cross-react with COX-1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Sh
Immunogen：Synthetic peptide corresponding to aa 567-599 (S567VPDPELIKTVTINASSSRSGLDDINPTVLLKE599) of human COX-2.
