Acyl-Coenzyme A is cholesterol acyltransferase-2 (ACAT-2) which catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A.
It is associated with lipoprotein particle secretion and thus plays an important role in the metabolism of triglyceride-rich lipoproteins like ApoB100.1,2,3 In humans, ACAT-2 is primarily expressed in the small intestine, whereas in other mammals, the enzyme is expressed in the liver and the small intestine.4,2 Human ACAT-2 has 522 amino acid with an estimated molecular weight of 60 kD.5 Detects ACAT-2 at about 60 kD in murine liver, intestine, and fibroblasts.6
Applications:Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 1:500, 1us/mlImmunohistochemistry: 1:500, 1ug/mlWestern Blot: 1:250, 2ug/ml for 1 hour at RT.
Overnight incubations at 4ºC with greater dilutions can produce optimal results
Optimal dilution to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ea
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.4, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ACAT-2. Species Crossreactivity: Mouse, rat, sheep and porcine.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Po Rt Sh
Immunogen：Synthetic peptide corresponding to aa3-20 (P3GGARLRLQRTEGLGGER20) of human ACAT-2
