The interaction between CD44 and hyaluronan may mediate binding of cells to basement membranes.
Several CD44 isoforms or variants arise from alternative splicing of mRNA transcribed from a single gene located on chromosome 11.
The alternatively spliced mRNA’s are translated into a family of protein isoforms which contain inserts in their cytoplasmic domains of varying lengths.
Most cell types express the standard CD44, while the expression of the isoforms resulting from the alternative splicing is of limited distribution.
CD44 isoforms resulting from alternative splicing have been detected in many human tumors; therefore, the identification of CD44 isoforms has utility in cancer studies.
Applications: Suitable for use in Immunohistochemistry and Western Blot.
Use alkaline phosphatase-anti-alkaline-phosphatase (APAAP) or peroxidase anti-peroxidase (PAP) procedure or the three stage immunoperoxidase technique on acetone fixed frozen sections.
Immunohistochemistry (Paraffin): 20ug/ml after 1x5 min microwave treatment
Use of biotinylated porcine anti-goat, mouse, rabbit immunogloulins is recommended.
Blocking should be done with porcine serum
Optimal conditions must be determined individually for each application.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3.
Specificity：Recognizes epitopes encoded by exons v3-v10 of human variant CD44.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human CD44var(v3-v10).
