Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes.
Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation.
Chemokines act through seven transmembrane G protein-coupled receptors.
The chemokine system is increasingly recognized to play an important role in lymphocyte biology.
CCR4 is a chemokine receptor and is preferentially expressed on type 2 helper T (Th2-type) cells.
In contrast to other chemokine receptors, the expression of CCR4 and CCR8 on Th2 cells is transiently increased following TCR and CD28 engagement.
Moreover, activated Th1 cells up-regulate CCR4 expression and functional responsiveness to thymus- and activation-regulated chemokine.
Applications:Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions: ELISA: 1:100,000Flow Cytometry: 1-3ul per 2x10e5 cells per 50ulWestern Blot: 1:1000Optimal dilution determined by the researcher.
Positive Control:Mouse spleen cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/ml BSA, 0.09% sodium azide.
Specificity：Recognizes mouse CCR4. Does not crossreact with human CCR4.
Isotype：F(ab’)2
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to aa8-26, D8TTQDETVYNSYYFYESMP26C) of the N-terminal extracellular domain of mouse CCR4 (C-C chemokine receptor 4).
