Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.
A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3).
Caspase 9 and Apaf-1 bind to each other, which leads to Caspase 9 activation (3).
Caspase 9 is also activated by granzyme B and CPP32 (1,2).
Activated Caspase 9 cleaves and activates Caspase 3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3).
Caspase 9 plays a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD95, FADD and TRADD (4).
Caspase 9 is expressed in a variety of human tissues (1,2).
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution: Western Blot: 0.5-4ug/mlOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Specificity：Recognizes human Caspase 9. Detects Procaspase 9 and a Caspase 9 cleavage product by Western Blot. Species Crossreactivity: mouse, rat, hamster, rabbit, porcine, canine, monkey and bovine.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Hm Hu Mk Mo Po Rb Rt
Immunogen：Synthetic peptide corresponding to aa287-306 of human Caspase 9.
