Caspase-3 (also know as CPP32, Yama and apopain) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases.
Caspase-3 exists in cells as an inactive 32kD proenzyme.
During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B.
The processed form of caspase-3 consists of large (17kD) and small (11kD) subunits which associate to form the active enzyme.
The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others.
The active recombinant caspase-3 is routinely tested for its ability to enzymatically cleave the caspase-3 colorimetric substrate Ac-DEVD-pNA.
Applications: Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): 1:10-1:20Western Blot: 1:50-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Specificity：Recognizes human Caspase 3, active. Does not cross-react with procaspase-3 (~32kD). Species Crossreactivity: mouse and rat active caspase-3 (p20 subunit).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide around the cleavage site of human caspase-3.
