Cadherins are transmembrane glycoproteins involved in Ca++ dependent cell adhesion (1).
The cadherin family, including (E)pithelial-Cadherin, (N)euronal-Cadherin, (P)lacental-Cadherin and (R)etinal-Cadherin, plays an important role in tissue morphogenesis and structure.
N-cadherin (136kD) is expressed in nerve cells, developing skeletal muscle, embryonic and mature cardiac muscle cells, and pleural mesothelial cells.
Studies have indicated that analysis of E-cadherin and N-cadherin expression may be useful in differentiating pleural mesotheliomas (N-cadherin positive) and lung adenocarcinomas (E-cadherin positive).
Applications: Suitable for use in Immunohistochemistry and Western Blot
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Frozen and paraffin): 5-10ug/mlWestern Blot: 5-10ug/mlOptimal dilution to be determined by researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：400ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA. Reconstitute with 200ul sterile ddH2O. Dilute with 20mM TBS, pH 7.4, 1% BSA, 0.1% sodium azide, 10mM calcium chloride. (PBS may cause precipitation). In case of immunohistochemistry on paraffin or frozen sections, Ca2+ is not necessary.
Specificity：Recognizes human N-cadherin. Species Crossreactivity: mouse, bovine and chicken. Does not cross-react with human E- or P-cadherin. Does not inhibit N-cadherin dependent cell-cell contact.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Ch Hu Mo
Immunogen：Synthetic peptide corresponding to aa808-827 of human N-cadherin.
